The activation of the ARE/Nrf2 pathway in keratinocytes is one of the key events in the complex cascade of skin sensitization. For the evaluation of potential sensitizers reporter gene cell lines, such as KeratinoSens®, have been validated (OECD 442D), which express luciferase upon Nrf2 activation.
The instaCELL® KeratinoSens® assay kit includes prequalified Assay Ready Cells, which can be seeded instantly after thawing in a 96-well plate without any further cultivation. The kit uses a multiplex protocol, where the viability (Resazurin) and Nrf2 activation (One-Glo™, Promega) is determined on the same cells in the same well. Technical proficiency of the kit is given through the correct determination of OECD recommended proficiency substances. The KeratinoSens® cell line uses the Luciferase technology from Promega (U.S. Pat. No. 8008006 & EU Pat. No. 1341808B1). The cells may only be used under the terms of a limited use license which is attached as part of this kit.