A possible genotoxic effect should be discovered as early as possible in the development of a new drug to avoid unnecessary costs. The instaCELL® micronucleus assay kit, based on OECD guideline 487, is a simple and quick method to determine the genotoxic potential of a candidate substance. The formation of micronuclei during cell division, caused by double-strand breaks, crosslinking etc., is used as a measure for the genotoxic damage. The cells are treated with the test substance directly on a microscope slide (3-well chamber, Ibidi®) and can be mounted and stained in one step (Fluoroshield™, ImmunoBioScience corp.), which makes the preparation very easy and convenient.
The instaCELL® micronucleus assay kit includes Assay Ready V79 Cells which can be used instantly after thawing like a reagent without prior cultivation. Due to the production of large homogeneous batches and storage in liquid nitrogen, the same high cell quality is available over years, which greatly increases the reproducibility of the assay. The cells have been prequalified for the assay and are provided in the kit together with medium, controls and the staining dye.