Pyrogens such as lipopoysaccharides can induce fever. The pyrogen potential of a substance can be detected by activation of macrophages (e.g. release of Interleukin-6). The instaCELL pyrogen detection kit is based on THP-1 cells which have been differentiated into CD11b and CD14 positive macrophages.
The kit includes assay ready macrophages. The cells can be used in the assay like a reagent without prior cultivation. They recover with high viability and adhere quickly. In addition to the assay ready cells, the kit includes plates, assay medium, standardized controls, and an ELISA read out for IL-6.