Skin Sensitization

Allergic contact dermatitis is an environmental health issue induced by chemicals which have been identified as skin sensitizers. Recently a set of alternative methods has been published by the OECD replacing the animal local lymph node assay. The cell based assays represent early key events of the skin sensitization process.

h-CLAT, the human Cell Line Activation Test and U-Sens using THP-1 or U937 cells which respond to skin sensitizes with the expression of CD54 and CD86 (OECD 442E). The instaCELL skin sensitization kit I contains all required reagents including assay ready THP-1 cells, which can be used for h‑CLAT without prior cultivation. The cells recover at high viability and reproducibly discriminate between sensitizers and non-sensitizers.

The keratinocyte cell lines KeratinoSens® and LuSens have been developed by Givaudan and BASF and express Luciferase reporter upon activation of oxidative stress pathways (OECD 442D). The cell lines are provided by acCELLerate.

instaCELL cytotoxcity assay kit

Skin Sensitization small

KeratinoSens (RE242)

1 vial keratinocyte ARE/Nrf2 reporter cell line (5E6 cells/vial)

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THP-1 assay ready cells (RE510)

5 vials (1E7 cells/vial), recovery buffer, assay medium

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U-937 assay ready cells (RE511)

5 vials (1E7 cells/vial), recovery buffer, assay medium

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