Allergic contact dermatitis is an environmental health issue induced by chemicals which have been identified as skin sensitizers. Recently a set of alternative methods has been published by the OECD replacing the animal local lymph node assay. The cell based assays represent early key events of the skin sensitization process.
h-CLAT, the human Cell Line Activation Test and U-Sens using THP-1 or U937 cells which respond to skin sensitizes with the expression of CD54 and CD86 (OECD 442E). The instaCELL skin sensitization kit I contains all required reagents including assay ready THP-1 cells, which can be used for h‑CLAT without prior cultivation. The cells recover at high viability and reproducibly discriminate between sensitizers and non-sensitizers.
The keratinocyte cell lines KeratinoSens® and LuSens have been developed by Givaudan and BASF and express Luciferase reporter upon activation of oxidative stress pathways (OECD 442D). The cell lines are provided by acCELLerate.