instaCELL skin sensitization assay kits

Allergic contact dermatitis is an environmental health issue induced by chemicals which have been identified as skin sensitizers. Recently a set of alternative methods has been punblished by OECD replacing the animal local lymph node assay. The cell based assays represent early key events of the skin sensitization process.

h-CLAT the human Cell Line Activation Test and U-Sens using THP-1 or U937 cells which respond to skin sensitizes with the expression of CD54 and CD86 (OECD 442E). The instaCELL skin sensitization kit I contain all required reagents including assay ready THP-1 cells, which can be used for h‑CLAT without prior cultivation. The cells recover at high viability and reproducibly discriminate between sensitizers and non-sensitizers.

The keratinocyte cell lines KeratinoSens® and LuSens have been developed by Givaudan and BASF and express Luciferase reporter upon activation of oxidative stress pathways (OECD 442D). The cell lines are provided by acCELLerate.



KeratinoSens (RE242)

1 vial keratinocyte ARE/Nrf2 reporter cell line (5E6 cells/vial)

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instaCELL skin sensitization assay kit I (h-CLAT) (SF210)

- THP-1 assay ready cells ( 4 vial)
- reagents, buffers and antibodies to perform 1x dose finding
  and 3x h-CLAT according to DB-ALM protocol N° 158

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THP-1 assay ready cells (RE510)

5 vials (1E7 cells/vial), recovery buffer, assay medium

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U937 assay ready cells (RE511)

5 vials (1E7 cells/vial), recovery buffer, assay medium

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