skin sensitization assays

Allergic contact dermatitis is an environmental health issue induced by chemicals which have been identified as skin sensitizers. Recently a set of alternative methods has been punblished by OECD replacing the animal local lymph node assay. The cell based assays represent early key events of the skin sensitization process.

h-CLAT the human Cell Line Activation Test and U-Sens using THP-1 or U937 cells which respond to skin sensitizes with the expression of CD54 and CD86 (OECD 442E). The instaCELL skin sensitization kit I contain all required reagents including assay ready THP-1 cells, which can be used for h‑CLAT without prior cultivation. The cells recover at high viability and reproducibly discriminate between sensitizers and non-sensitizers.

The keratinocyte cell lines KeratinoSens® and LuSens have been developed by Givaudan and BASF and express Luciferase reporter upon activation of oxidative stress pathways (OECD 442D). The cell lines are provided by acCELLerate.



instaCELL skin sensitization assay kit I (h-CLAT) (SF210)

- THP-1 assay ready cells ( 4 vial)
- reagents, buffers and antibodies to perform 1x dose finding
  and 3x h-CLAT according to DB-ALM protocol N° 158

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KeratinoSens® (RE242)

- ARE/Nrf2 reporter keratinocyte cell line
- 5 million cells/vial (cyropreserved)
- for use according to DB-ALM protocol N° 155
- provided under license from Givaudan

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THP-1 assay ready cells (RE510-05)

human monocytic leukemia cells
5 vials (1E7 cells/vial), recovery buffer, assay medium

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U937 assay ready cells (RE511-05)

human monocytic leukemia cells
25 vials (1E7 cells/vial), recovery buffer, assay medium

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