instaCELL® KeratinoSens® assay kit
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Assay Ready vs. Continious Culture
The activation of the ARE/Nrf2 pathway in keratinocytes is one of the key events in the complex cascade of skin sensitization. For the evaluation of potential sensitizers, reporter gene cell lines such as KeratinoSens® have been validated (OECD 442D), which express luciferase upon Nrf2 activation.
Assay Ready KeratinoSens® Cells were qualified for the test in comparison with KeratinoSens® cells from a continious culture. The reference skin sensitizer EGDMA did not reveal any altered sensitivity in terms of viability (blue) or Nrf2 activation (red).
![Keratinosens-Kit Keratinosens-Kit](/fileadmin/_processed_/9/6/csm_keratinosens-kit_web_963f1684f0.jpg)
![Skin Sensitization](/fileadmin/_processed_/7/b/csm_Skin_Sensitization_796d5efa56.jpg)