The instaCELL GM-CSF potency assay kit is an in vitro test to determine the potency of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) formulations for quality control. Recombinant forms of GM-CSF are used for white blood cell recovery following autologous bone marrow transplantation or chemotherapy and for the treatment of fungal infections. The assay utilizes the cytokine dependence of TF-1 cells, a human erythroblast cell line, for their proliferation, which make them a valid model to determine the potency of cytokines like GM‑CSF in a proliferation bioassay. The assay kit uses a Resazurin viability assay to analyze the potency of the GM-CSF product and compares it to a kit included reference.
The instaCELL G-CSF assay kit includes prequalified TF-1 instaCELLs as well as media, reagents, reference standard, and 96-well plate. InstaCELLs are frozen cell aliquots which can be used directly in the assay without prior cultivation, basically like a reagent.